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Introducing 50 kb DNA Synthesis

Let us do the assembly for you.

Ansa’s 50 kb DNA synthesis offering marks a new era in synthetic biology, where DNA length is no longer a constraint on what scientists can design or build. Whether you’re building a new chassis organism, designing an entire viral vector from scratch, or scaling up from lab bench to biomanufacturing, Ansa’s long DNA capabilities ensure you won’t be held back by synthesis constraints.

  • Market-Changing Pricing
    Ansa offers a continuously decreasing price curve from 7.5 kb to 50 kb, meaning the cost per base pair drops as DNA length increases. And with pricing as low as $0.28/bp, large-scale projects are now more affordable than ever.
  • Speed That Accelerates Discovery
    With standard turnaround times starting at 25 business days, you’re equipped to move faster from design to experimentation.
  • Unrivaled Quality You Can Trust
    Every construct is fully sequence verified and QC-tested, so you receive reliable, ready-to-use DNA. No surprises, no delays.
  • Proudly Made in the USA
    Our DNA synthesis, assembly, and quality control all happen domestically, ensuring the highest standards.
XL CLONAL DNA (7.5 kb - 50 kb)
DNA sequence length 7,501 bp - 20,000 bp 20,001 bp - 30,000 bp 30,001 bp - 40,000 bp 40,001 bp - 50,000 bp
GC Content 10% - 94%
A or T homopolymers ≤ 140 bp
G or C homopolymers ≤ 16 bp
Short tandem repeats ≤ 10 repeat units
Inverted repeats ≤ 50 bp per side
Error rate Clonal
Typical yield 1.5 µg
Vector pStable-Ind-Chlor-v1.1
Scale-up 10 µg–1 mg of DNA per prep, Standard or Endotoxin-free
Pricing (variable/bp)
Guaranteed Shipping In as little as 25 business days
XL CLONAL DNA (7.5 kb - 50 kb)
GC Content 10% - 94%
A or T homopolymers ≤ 140 bp
G or C homopolymers ≤ 16 bp
Short tandem repeats ≤ 10 repeat units
Inverted repeats ≤ 50 bp per side
Error rate Clonal
Typical yield 1.5 µg
Vector pStable-Ind-Chlor-v1.1
Scale-up 10 µg–1 mg of DNA per prep, Standard or Endotoxin-free
Pricing (variable/bp) $0.38/bp - $0.28/bp
Guaranteed Shipping In as little as 25 business days

All products are manufactured in the USA and shipped globally. Biosecurity screening is done in accordance with the guidance of US Department of Health and Human Services’ Screening Framework Guidance for Providers and Users of Synthetic Nucleic Acids before being synthesized.

Let us get you the complex DNA you need to achieve new scientific breakthroughs.

XL Clonal Vector Map & Sequence

Ansa’s XL Clonal DNA uses a specific vector optimized for long, complex sequences. Download the vector map and sequence.

I’m interested in the XL Clonal DNA and my sequence is longer than 7.5 kb. Which vectors are available for >7.5 kb sequences?

We provide 7,501 bp – 50,000 bp in pStable-Ind-Chlor-v1.1, a single-copy vector that can be induced to mid-copy number using arabinose. Very large plasmids can be difficult to propagate in E. coli using higher copy vectors. This backbone ensures more reliable propagation during cell growth while enabling you to obtain a much higher yield than you could obtain with a single-copy vector. This higher yield is achieved by inducing the copy number after most of the cell growth has already happened.

Download sequence file | Download vector map

What timeline can I expect my project to be provided?

We aim for competitive turnaround times (TAT) depending on your size and format.

  • DNA fragments (200–900 bp) ship in as few as 4 business days.
  • Clonal DNA (100 bp–7.5 kb) ships in as little as 11 business days.
  • XL Clonal DNA (7.5 kb–50 kb) ships in as few as 25 business days.

Shipment times are dependent on when your order is placed. Reach out to your account manager or email us at customerservice@ansabio.com for more details.

What exactly does the Ansa On-Time Guarantee (AOTG) Program guarantee? 

AOTG guarantees that your order will be completed and shipped on or before the expected ship date. If any part of your order fails to meet this date for DNA Fragments and Clonal DNA (100 bp – 7.5 kb) orders, the entire order is free—no exceptions.

For larger orders, such as XL Clonal DNA (7.5 kb–50 kb), Ansa may handle the order in separate groups, each with its own guaranteed shipping schedule. The sequences in each group will be assigned a guaranteed shipping date, as applicable. This ensures customers can confidently plan their work, reducing the uncertainty associated with legacy DNA synthesis vendors. It underscores Ansa’s confidence in its technology and its commitment to reliability and customer satisfaction.

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“Our experience with Ansa Biotechnologies has been excellent. The company successfully delivered a set of complex, repetitive sequences up to 41 kb that several other companies had declined to synthesize, all in about half the time of current industry standards. The Ansa team is responsive, easy to work with, and consistently follows through.”

George Church, PhD
Professor, Harvard Medical School and Massachusetts Institute of Technology

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“Ansa has been a consistent, responsive, and reliable partner who Terrain has utilized to great effect to deliver us highly complex DNA constructs that enable us to build complex and long mRNA sequences. As the proteins used in medicine get larger and more complex, so too do the sequences that encode them. Our partnership with Ansa, and in particular their 7.5kb - 50kb clonal gene offering enables us to push the limits of mRNA design and manufacturing. It has been a pleasure working with Ansa and it always feels like a true partnership.”

Aaron Larsen, PhD
Chief Scientific Officer, Terrain Bio

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“Ansa has provided us with extremely long and complex constructs we couldn't get anywhere else. For these types of sequences, as long as 50 kb, other providers often reject or quote an extremely long ETA. Without Ansa, we couldn't obtain them so efficiently, affordably, and reliably - or at all!”

Molly Gasperini, PhD
Associate Director of Genome Engineering
Allen Institute’s Seattle Hub for Synthetic Biology

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“Long and complex DNA constructs are essential for our transgenic animal model development work. It is time-consuming to build these long constructs in-house. Ansa delivered a 23 kb construct to us in record time, even though we were one of the first customers in their early access program. We're excited about how reliable access to long and complex sequences can accelerate our model generation capabilities.”

Piotr Bobrowicz, PhD
President, Alloy Therapeutics

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“The full-length 50kb fragment integrated into the targeted human genome locus on our first try! Thank you all again for the support and extra help on the DNA prep, which saved our team a lot of time.”

Simon Wu, PhD
Director, Project Management and Technical Operation, Applied StemCell

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Start Unlocking Breakthroughs

The future of synthetic biology starts at 50 kb. Expand your design space and submit your sequences today.