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Direct, Sequence-Perfect Clonal DNA Synthesis from 100 bp – 7.5 kb

At Ansa, we pride ourselves on delivering sequence-verified clonal DNA that other providers often cannot produce. Powered by our proprietary enzymatic DNA synthesis platform, we enable the direct synthesis of complex DNA sequences with industry-leading accuracy and consistency. Our approach is designed to handle a wide range of sequence complexity, making it ideal for advanced synthetic biology and molecular biology applications.

With unmatched precision, Ansa is the only provider offering direct, contiguous synthesis of 900 bp constructs without cloning or assembly. This enables researchers to move faster with error rate–controlled, high-fidelity DNA that is ready for downstream use.

Additionally, Ansa now offers a variety of Catalog Vectors optimized for clonal DNA applications. These vectors support rapid construction of expression vectors, plasmid-based constructs, and other DNA constructs critical to modern research. Explore the catalog to see the available options, or learn about our early access program for custom designs.

CLONAL DNA (100 bp - 7.5 kb)
DNA sequence length 100 bp - 1,500 bp 1,501 bp - 3,500 bp 3,501 bp - 5,500 bp 5,501 bp - 7,500 bp
GC Content 10% - 94%
A or T homopolymers ≤ 140 bp
G or C homopolymers ≤ 16 bp
Short tandem repeats ≤ 10 repeat units
Inverted repeats ≤ 50 bp per side
Error rate Clonal
Typical yield 1.5 µg
Vector Choose from our Catalog Vectors
Scale-up 10 µg–1 mg of DNA per prep, Standard or Endotoxin-free
Standard Pricing $195 per construct $0.13/bp $0.17/bp $0.28/bp
Complex Pricing $375 per construct $0.25/bp $0.35/bp $0.38/bp
Guaranteed Shipping In as little as 11 business days
CLONAL DNA (100 bp - 7.5 kb)
GC Content 10% - 94%
A or T homopolymers ≤ 140 bp
G or C homopolymers ≤ 16 bp
Short tandem repeats ≤ 10 repeat units
Inverted repeats ≤ 50 bp per side
Error rate Clonal
Typical yield 1.5 µg
Vector Choose from our Catalog Vectors
Scale-up 10 µg–1 mg of DNA per prep, Standard or Endotoxin-free
Standard Pricing $0.13/bp - $0.28/bp
Complex Pricing $0.25/bp - $0.38/bp
Guaranteed Shipping In as little as 11 business days

All products are manufactured in the USA and shipped globally. Biosecurity screening is done in accordance with the guidance of US Department of Health and Human Services’ Screening Framework Guidance for Providers and Users of Synthetic Nucleic Acids before being synthesized.

Let us get you the complex DNA you need to achieve new scientific breakthroughs.

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What is custom gene synthesis?

Custom gene synthesis is the process of designing and producing synthetic DNA sequences tailored to specific research needs. Unlike traditional cloning methods that rely on biological templates, gene synthesis allows precise control over sequence design, codon usage, and functional elements with exceptional accuracy.

How does Ansa’s gene synthesis differ from traditional providers?

Ansa uses enzymatic DNA synthesis, enabling accurate construction of DNA fragments, clonal DNA, and ultra-long sequences up to 50 kb. Unlike other DNA synthesis providers, Ansa’s technology is based on TdT-dNTP conjugates—specialized enzymatic reagents that allow rapid and controlled single-base additions to a DNA molecule. This innovative approach overcomes the limitations of chemical synthesis and was designed for exceptional accuracy, speed, and reliability.

Who uses custom gene synthesis services?

Researchers in synthetic biology, cell and gene therapy, antibody discovery, agricultural biology, and many other application areas use custom gene synthesis to build accurate DNA constructs for research and product development.

Why choose a custom gene synthesis provider like Ansa?

Ansa’s innovative enzymatic DNA synthesis technology offers key advantages over both chemical and other enzymatic methods. Our technology is:

  • Capable of synthesizing long and complex DNA sequences with high accuracy
  • Fully enzymatic and does not damage DNA during synthesis
  • Optimized for speed and reliability, ensuring faster delivery and dependable results

By choosing Ansa, you gain access to breakthroughs in projects that are limited by conventional synthesis methods.

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“We are very excited about the Ansa technology, which has derisked timelines for creating our TARGATT™ donor plasmids," said Dolores Baksh, PhD, CEO of Applied StemCell. "Ansa's large DNA synthesis capabilities align well with the strength of our TARGATT™ technology, which efficiently and site-specifically inserts multi-protein constructs into the H11 safe harbor site in mammalian cells. We find that Ansa can even do difficult and complex sequences that can be challenging to clone or synthesize, and the inserted DNA always expresses well.”

Dolores Baksh, PhD
CEO, Applied StemCell

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“We requested a few [sequences] that we couldn’t synthesize otherwise, and got them through this very first shipment. Cloning went smoothly and the sequences look perfect. Thanks to @AnsaBio!”

Xiaojing Gao, PhD
Assistant Professor, Stanford University

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“The construct was perfect- exactly what I needed, and I could not have gotten this to work without your help.”

Liyam Chitayat
Hertz Fellow and PhD Student
Massachusettes Institute of Technology, Boyden Lab

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Move your research forward by letting us synthesize the complex sequences you need.