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DNA Antibody Grammar Language Case Study
Learn about Protein Coding Sequence grammar – subtle patterns in codon choice, repeats, and GC content shape how efficiently a cell can read and produce them.
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DNA Synthesis for the AI Era
This comprehensive guide explores how enzymatic DNA synthesis is rewriting the playbook for reliability, sequence complexity, and speed.
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Expanding the Capabilities of Targeted Integration
Ansa Bio and Applied StemCell partner to successfully deliver a 50 kb multi-cassette knock-in at a precise location in the human genome using their TARGATT™ platform and Ansa’s DNA synthesis technology. 
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Overcoming Critical DNA Synthesis Challenges in Viral Vector-Mediated Gene Therapy Development
Ansa’s proprietary enzymatic DNA synthesis overcomes the challenges in synthesizing viral vector DNA constructs – specifically inverted terminal repeats and regulatory sequences – enabling faster and more reliable development of viral gene therapies.
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Unlock Breakthroughs with Ansa Clonal DNA and Ansa DNA Fragments
Ultra-rapid, enzymatic synthesis enables the delivery of complex DNA sequences up to 50 kb.
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Access More Sequences with Ansa Biotechnologies
Ansa’s DNA synthesis approach has the industry’s lowest error rate, which allows us to directly create 900 bp oligonucleotides. These long oligonucleotides enable the generation of DNA with complex features that cannot be constructed by assembling short oligonucleotides.
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DNA Synthesis Needs A Paradigm Shift
To accelerate research, scientists need longer and more complex DNA sequences than traditional synthesis techniques can build.
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Rapid Manufacturing of Long, Complex DNA Constructs Using Sequence-Agnostic Enzymatic Synthesis
When considering downstream gene expression, the best codon selection is not necessarily the easiest DNA sequence to build via traditional chemical synthesis. With Ansa’s sequence-agnostic enzymatic synthesis, compromising downstream optimization is not necessary.
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Revolutionizing Gene Therapies through Rapid, Large DNA Synthesis & Designable Delivery Vehicles
Ansa Bio and EndoFold share results from a proof-of-concept collaboration demonstrating a new path forward: ultra-rapid synthesis of full-length therapeutic constructs paired with next-generation, designable delivery vehicles.
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Site-specific Integration of a 50 kb Multi-gene Payload Into Human iPSCs
Applied StemCell and Ansa Biotechnologies demonstrate the site-specific integration of a 50 kb multi-gene payload into human iPSCs, enabling advanced engineering of next-generation cell therapy platforms. The work highlights efficient safe-harbor integration and functional expression of complex genetic constructs for cell therapy applications. 
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A Complete Protein Suite to Program Compact Secretion
The Gao Lab at Stanford University has expanded the capabilities of traditional gene circuits into secretion protein circuits. This poster describes how they developed a suite of protein engineering approaches to enable this level of programmability completely at the protein level, with potential uses in AAV vectors and mRNA for use in transient therapeutics.
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Fully Synthetic Chassis for the Rapid Engineering of Mycobacteriophages with Therapeutic Potential
This poster from the Lohman Lab at NEB describes success with phage engineering using synthetic mycobacteriophages. The cloned and sequence-verified parts permitted easy generation of high-quality DNA for Golden Gate assembly and the insertion of anti-defense genes, laying the groundwork for therapeutics against hospital-acquired mycobacterial infections.
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Synthesis and Propagation of Complex AAV Gene-of-Interest Plasmids
Synthesis and propagation of plasmid DNA molecules with complex sequence motifs is an established pain point in manufacturing genetic medicines. A groundbreaking enzymatic DNA synthesis platform from Ansa Biotechnologies in conjunction with Novel Bio’s in-house DNA assembly workflow reliably synthesized and produced complex plasmid DNAs used in the production of viral vectors for gene therapy.
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Application of High Complexity Golden Gate Assembly to Rapid Engineering of Bacteriophage Genomes
Data-optimized Assembly Design (DAD) enhances Golden Gate Assembly (GGA) by using precise ligation fidelity measurements to select high-accuracy fusion sites. This approach replaces traditional GGA methods, enabling the efficient assembly of multiple parts while minimizing errors.
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