Vector Library for Cloning, Mammalian, AAV & Transcription Applications
Ansa now offers a variety of catalog vectors for your clonal constructs. We have options for cloning, bacterial, mammalian, AAV, and transcription applications, featuring several preferred vectors most commonly used by our customers.
The standard choices for sequences up to 7.5 kb are pANSA-Amp-v1.5 and pANSA-Kan-v1.5. Our most reliable vectors for long, complex, or toxic sequences up to 7.5 kb are pStable-Ind-Amp-v1.0 and pStable-Ind-Kan-v1.0.
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Learn about our custom vector early access program to onboard exactly what you need.
Catalog Vectors
Catalog vectors are designed to integrate seamlessly into your downstream cloning and expression workflows. Single vectors can support workflows from basic cloning to high-level protein expression depending on the promoter and backbone included. Vectors download maps and sequence files are available so you can plan cloning strategies, design primers, and simulate expression outcomes before ordering.
* Most commonly used vector
In vitro transcription vector including a truncated T7 promoter and a segmented 120bp polyA sequence. For functional expression initiation bases must be added to the start of the insert sequence (GN or AG depending on selected capping reagents). More detailed information is provided in a technical note here. Copy Number: High | Transcription vector |  | ||
In vitro transcription vector including a truncated T7 promoter and a segmented 120bp polyA sequence. For functional expression initiation bases must be added to the start of the insert sequence (GN or AG depending on selected capping reagents). More detailed information is provided in a technical note here. Copy Number: High | ||||
Ansa's most reliable vector for delivering complex and toxic sequences. The copy number remains 1-2 copies/cell until copy number induction with arabinose. Download the protocol. Copy Number: Inducible | Cloning |  | ||
Ansa's most reliable vector for delivering complex and toxic sequences. The copy number remains 1-2 copies/cell until copy number induction with arabinose. Download the protocol. Copy Number: Inducible | ||||
Ansa's most reliable vector for delivering complex and toxic sequences. The copy number remains 1-2copies/cell until copy number induction with arabinose. Download the protocol. Copy Number: Inducible | Cloning |  | ||
Ansa's most reliable vector for delivering complex and toxic sequences. The copy number remains 1-2copies/cell until copy number induction with arabinose. Download the protocol. Copy Number: Inducible | ||||
Bacterial protein expression vector under the control of a strong T7 promoter. A start and stop codon must be added to mediate start and stop of translation. The included lacI gene prevents leaky expression, that is relieved upon addition of IPTG. Transcription termination is mediated by an included T7 terminator. Requires a host strain that harbors a T7 RNA polymerase cassette (eg. (DE3)-containing strains) for expression. Copy Number: Medium | Bacterial Expression |  | ||
Bacterial protein expression vector under the control of a strong T7 promoter. A start and stop codon must be added to mediate start and stop of translation. The included lacI gene prevents leaky expression, that is relieved upon addition of IPTG. Transcription termination is mediated by an included T7 terminator. Requires a host strain that harbors a T7 RNA polymerase cassette (eg. (DE3)-containing strains) for expression. Copy Number: Medium | ||||
Bacterial protein expression vector under the control of a strong T7 promoter. A start and stop codon must be added to mediate start and stop of translation. The included lacI gene prevents leaky expression, that is relieved upon addition of IPTG. Transcription termination is mediated by an included T7 terminator. Requires a host strain that harbors a T7 RNA polymerase cassette (eg. (DE3)-containing strains) for expression. Copy Number: Medium | Bacterial Expression |  | ||
Bacterial protein expression vector under the control of a strong T7 promoter. A start and stop codon must be added to mediate start and stop of translation. The included lacI gene prevents leaky expression, that is relieved upon addition of IPTG. Transcription termination is mediated by an included T7 terminator. Requires a host strain that harbors a T7 RNA polymerase cassette (eg. (DE3)-containing strains) for expression. Copy Number: Medium | ||||
Commonly used high-copy, pMB1 origin cloning vector. Copy Number: High | Cloning |  | ||
Commonly used high-copy, pMB1 origin cloning vector. Copy Number: High | ||||
Commonly used high-copy, pMB1 origin cloning vector. Copy Number: High | Cloning |  | ||
Commonly used high-copy, pMB1 origin cloning vector. Copy Number: High | ||||
Medium-copy cloning vector utilizing a modified pMB1 origin of replication. Copy Number: Medium | Cloning |  | ||
Medium-copy cloning vector utilizing a modified pMB1 origin of replication. Copy Number: Medium | ||||
Medium-copy cloning vector utilizing a modified pMB1 origin of replication. Copy Number: Medium | Cloning |  | ||
Medium-copy cloning vector utilizing a modified pMB1 origin of replication. Copy Number: Medium | ||||
Transgene expression vector with AAV2 serotype ITRs flanking a CMV promoter, WPRE enhancer, and SV40 polyA signal to enable packaging and transgene expression in AAV. A kozak sequence, start, and stop codon must included in the insert region for transgene expression. Copy Number: High | AAV |  | ||
Transgene expression vector with AAV2 serotype ITRs flanking a CMV promoter, WPRE enhancer, and SV40 polyA signal to enable packaging and transgene expression in AAV. A kozak sequence, start, and stop codon must included in the insert region for transgene expression. Copy Number: High | ||||
Transgene expression vector with AAV2 serotype ITRs flanking a CMV promoter, WPRE enhancer, and SV40 polyA signal to enable packaging and transgene expression in AAV. A kozak sequence, start, and stop codon must included in the insert region for transgene expression. Copy Number: High | AAV |  | ||
Transgene expression vector with AAV2 serotype ITRs flanking a CMV promoter, WPRE enhancer, and SV40 polyA signal to enable packaging and transgene expression in AAV. A kozak sequence, start, and stop codon must included in the insert region for transgene expression. Copy Number: High | ||||
Transient or stable mammalian expression vector under the control of a strong CMV enhancer & promoter. Downstream of the insert is a bGH polyA signal to mediate transcription stop. A neomycin marker is present to enable G-418 (Geneticin) selection for stable cell line engineering. A kozak sequence, start, and stop codon must included in the insert region for protein expression Copy Number: Medium | Mammalian Expression |  | ||
Transient or stable mammalian expression vector under the control of a strong CMV enhancer & promoter. Downstream of the insert is a bGH polyA signal to mediate transcription stop. A neomycin marker is present to enable G-418 (Geneticin) selection for stable cell line engineering. A kozak sequence, start, and stop codon must included in the insert region for protein expression Copy Number: Medium | ||||
Custom Vector Onboarding Early Access
Jump-start your research with Ansa’s vector onboarding, now available in Early Access. Our new program removes the traditional pain points of plasmid onboarding, letting you focus on discovery, not logistics.
- No Shipping Required: Eliminate the need to send physical DNA or plasmids. Simply upload your sequence file and Ansa will synthesize your entire vector.
- Seamless, Hassle-Free Workflow: We deliver ready-to-use plasmids straight out of the tube, saving you time and effort.
- Single Source: Ansa is your single provider for standard and complex DNA synthesis, and plasmid construction – no need to juggle multiple vendors.
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